The Burrill Report


Scientists have made an important advance in understanding how some bacterial cells escape being killed by many antibiotics—they become “persisters” by entering a state in which they stop replicating and are able to tolerate antibiotics. While antibiotic resistance is due to genetic mutations and is passed on to later generations, this tolerant phase is only temporary, but it may contribute to the later development of resistance.

Virtually all bacterial species form subpopulations of persisters that are tolerant to many antibiotics. Persisters are likely to be a cause of many recurrent infections, but little is known about how they arise.

“We rely on antibiotics to defend us against common bacterial infections like tuberculosis, cystitis, tonsillitis and typhoid, but a few cells can escape treatment by becoming persisters, which allows the infection to come back,” says Sophie Helaine, a co-lead author of the study. “This is a big problem in itself, but it also makes it more likely that antibiotic resistance will arise and spread.”

In a new study in the journal Science, researchers from the MRC Centre for Molecular Bacteriology and Infection at Imperial College London have succeeded in visualizing persister cells in infected tissues for the first time, and have identified signals that lead to their formation.

The team developed a method for tracking single cells using a fluorescent protein produced by the bacteria. They showed that Salmonella, which causes gastroenteritis and typhoid fever, forms large numbers of non-replicating persisters after being engulfed by immune cells called macrophages. By adopting this non-replicating mode, Salmonella survives antibiotic treatment and lingers in the host, accounting for its ability to cause recurrent infections.

The researchers also identified factors produced by human cells that trigger bacteria to become persisters. “One of the most striking findings in this work is that conditions inside immune cells activate two different responses from Salmonella, causing some bacteria to replicate and others to enter a non-replicating persister state,” says David Holden, co-lead author and director of the MRC Centre for Molecular Bacteriology and Infection at Imperial College London. “Activating these two responses together is likely to be an important mechanism by which Salmonella survives during infection.”

Now that they know the molecular pathways and mechanisms that lead to persister formation during infection, the researchers can work on screening for new drugs to coax them out of this state so that they become vulnerable to antibiotics, Helaine says.

The research was supported by an Imperial College London Junior Research Fellowship, the Wellcome Trust and the Medical Research Council.

January 10, 2014
http://www.burrillreport.com/article-new_clues_as_to_how_bacteria_evade_antibiotics.html